Add staircase-ramp protocol

Author: chonlei

README.md

@@ -1,17 +1,38 @@
-# Rapid Characterisation Protocols for patch-clamp electrophysiology
+# Rapid Characterisation Protocols for Patch-Clamp Electrophysiology
+
 Collection of protocols for rapid characterisation of ion channel kinetics.
 
 Here we have provided plain text `.txt` and comma-separated-value `.csv` formats.
 
 `.atf` files are also provided and are intended to be used with the Axon pClamp software.
 
+Some also support `.eph` files which are intended to be used with the Nanion SyncroPatch software.
+
+## Staircase voltage clamp for hERG/IKr measurements
+The protocol `staircase-ramp` is a short, high information-content voltage clamp protocol that is applicable in _automated high-throughput patch clamp systems_.
+The protocol and its application have a dedicated [Github repository](https://github.com/CardiacModelling/hERGRapidCharacterisation)).
+
 ## Sinusoidal voltage clamp for hERG/IKr measurements
+The protocol `sinewave-ramp` is a slightly-updated version of the sinusoidal voltage clamp we first published in 2018 (which has a dedicated [Github repository](http://www.github.com/mirams/sine-wave)).
+The update here is a ramp during the initial 'leak step' rather than a fixed potential.
 
-The first protocol is `sinewave-ramp` which is a slightly-updated version of the sinusoidal voltage clamp we first published in 2018 (which has a dedicated [Github repository](http://www.github.com/mirams/sine-wave)). The update here is a ramp during the initial 'leak step' rather than a fixed potential.
 
 # Format/Units
+
 Each file has two columns, the first one is time (in [seconds]) and the second column is voltage (in [milliVolts]).
 
+
 # Acknowledging this work
-If you publish any work based on the contents of this repository please cite:  
-Beattie, K. A., Hill, A. P., Bardenet, R., Cui, Y., Vandenberg, J. I., Gavaghan, D. J., de Boer, T. P. & Mirams, G. R. [Sinusoidal voltage protocols for rapid characterisation of ion channel kinetics](https://doi.org/10.1113/JP275733). J. Physiol. 596, 1813–1828 (2018).
+
+If you publish any work based on the contents of this repository please cite:
+
+Lei, C. L., Clerx, M., Gavaghan, D. J., Polonchuk, L., Mirams, G. R., Wang, K.
+(2019).
+[Rapid characterisation of hERG channel kinetics I: using an automated high-throughput system](https://doi.org/10.1101/609727).
+_bioRxiv_, 609727.
+
+Beattie, K. A., Hill, A. P., Bardenet, R., Cui, Y., Vandenberg, J. I., Gavaghan, D. J., de Boer, T. P. & Mirams, G. R.
+(2018).
+[Sinusoidal voltage protocols for rapid characterisation of ion channel kinetics](https://doi.org/10.1113/JP275733).
+_J. Physiol._ 596, 1813–1828.
+

sinewave-ramp.csv

@@ -1,3 +1,4 @@
+"time","voltage"
 0.000000000000000000e+00,-8.000000000000000000e+01
 1.000000000000000048e-04,-8.000000000000000000e+01
 2.000000000000000096e-04,-8.000000000000000000e+01