[Bug]: PYNWB_VALIDATION when using ndx-miniscope extension

[weiglszonja]

What happened?

When running nwbinspector on a file that uses ndx-miniscope to add Miniscope device, it flags a PYNWB_VALIDATION for the ImagingPlane as:

1  PYNWB_VALIDATION
===================

1.1  /Users/weian/catalystneuro/demo_notebooks/C6-J588-Disc5.nwb: ImagingPlane - 'None' object at location 'general/optophysiology/ImagingPlane'
       Message: missing data type Device (device)

However the device looks correctly linked to ImagingPlane:

Operating System

M1 macOS

Python Version

3.9

Were you streaming with ROS3?

No

Package Versions

nwbinspector 0.4.30
ndx-miniscope 0.5.1

Code of Conduct

• I agree to follow this project's Code of Conduct
• Have you ensured this bug was not already reported?
• To the best of your ability, have you ensured this is a bug within the code that checks the NWBFile, rather than a bug in the NWBFile reader (e.g., PyNWB or MatNWB)?

[weiglszonja]

We just discussed with @bendichter that this is more likely a pynwb validation error, I'll open an issue there.

[CodyCBakerPhD]

@weiglszonja I just went ahead and transferred it

[CodyCBakerPhD]

The ndx-miniscope extension defines a custom Device here: https://github.com/catalystneuro/ndx-miniscope/blob/main/spec/ndx-miniscope.extensions.yaml#L2-L4

io.write works fine, even io.read; but it seems pynwb.validate does not like the subtype of Device linked to the ImagingPlane?

@weiglszonja Can you share the full traceback of pynwb.validate and figure a way to share the file easily with @rly or @oruebel?

[oruebel]

When you do io.read what does nwbfile.get_imaging_plane('ImagingPlane').device return? I'm just wondering, because the validator seems to indicate None.

[weiglszonja]

@oruebel @CodyCBakerPhD Thank you for helping figuring out this issue,

nwbfile.get_imaging_plane("ImagingPlane").device

returns

Miniscope abc.Miniscope at 0x5828172864
Fields:
  compression: FFV1
  deviceType: Miniscope_V3
  frameRate: 15FPS
  framesPerFile: 1000
  gain: High
  led0: 47

I just tried this on the file:

from pynwb import validate

nwbfile_path = "C6-J588-Disc5.nwb"
print(validate(paths=[nwbfile_path], verbose=True))

returns

Validating /Users/weian/catalystneuro/demo_notebooks/C6-J588-Disc5.nwb against cached namespace information using namespace 'ndx-miniscope'.
([], 0)

So the file passed the validation then?
But running nwbinspector again:

import nwbinspector

nwbfile_path = "C6-J588-Disc5.nwb"
print(list(nwbinspector.inspect_nwbfile(nwbfile_path))[0])

returns

InspectorMessage(
    message='missing data type Device (device)',
    importance=<Importance.PYNWB_VALIDATION: 3>,
    severity=<Severity.LOW: 1>,
    check_function_name='ImagingPlane',
    object_type=None,
    object_name=None,
    location='general/optophysiology/ImagingPlane',
    file_path='/Users/weian/catalystneuro/demo_notebooks/C6-J588-Disc5.nwb'
)

[CodyCBakerPhD]

from pynwb import validate

nwbfile_path = "C6-J588-Disc5.nwb"
print(validate(paths=[nwbfile_path], verbose=True))

The call pattern in the Inspector is actually slightly different; https://github.com/NeurodataWithoutBorders/nwbinspector/blob/dev/src/nwbinspector/nwbinspector.py#L555

Can you try again using the classical way of doing the validation?

with NWBHDF5IO(...) as io:
    pynwb.validate(io=io)

[weiglszonja]

you're right @CodyCBakerPhD !

from pynwb import validate
from pynwb import NWBHDF5IO
nwbfile_path = "C6-J588-Disc5.nwb"

with NWBHDF5IO(nwbfile_path, load_namespaces=True) as io:
    print(validate(io=io, verbose=True))

returns

[ImagingPlane (general/optophysiology/ImagingPlane): missing data type Device (device)]

[rly]

@weiglszonja could you please upload the file to https://drive.google.com/drive/u/0/folders/197eVWGgqSItz5DethQdEjlyw_-iCyRSY if you can, so that I can troubleshoot? Thanks.

Strange that

from pynwb import validate

nwbfile_path = "C6-J588-Disc5.nwb"
print(validate(paths=[nwbfile_path], verbose=True))

and

from pynwb import validate
from pynwb import NWBHDF5IO
nwbfile_path = "C6-J588-Disc5.nwb"

with NWBHDF5IO(nwbfile_path, load_namespaces=True) as io:
    print(validate(io=io, verbose=True))

have different results. I suspect that one is not loading the namespaces correctly.

[weiglszonja]

Thank you @rly , I uploaded a small file.

[rly]

Code snippet 1) above results in no validation errors - the correct behavior - because validate by default will validate against all cached namespaces and ignore dependent namespaces. In this case, the only namespace that will be validated against is ndx-miniscope.

Code snippet 2) above results in a validation error because it only validates against a namespace arg if provided or the core namespace otherwise. In this case, it will validate against the core namespace, and that validation appears not to understand that data type Miniscope extends data type Device. Adding a namespace arg -- print(validate(io=io, verbose=True, namespace="ndx-miniscope")) -- results in no validation errors like in case 1.

This raises a few questions.

1. What should the default behavior be for validate when the io arg is passed? I think it should be changed to the same default behavior as when the paths arg is passed where the IO object is validated against all cached namespaces except for dependent ones. It looks like when we last updated pynwb.validate, we decided to keep the existing behavior of validate with io passed to maintain backward compatibility with previous versions of pynwb. Generalize Validation #1511 (comment) . The new split behavior is confusing and I think it would be OK to change this behavior.

2. Why does validation on the core namespace not understand the type of the Miniscope device? "ndx-miniscope" was loaded. It looks like in ValidatorMap, only the types in the given namespace are processed. However, this will result in problems when there are two extensions A and B that independently extend the core namespace. Validation of the file on namespace A will not know about data types in B and vice versa.

3. Is there any use case for not simply validating on all the loaded namespaces together? Perhaps both ValidatorMap and pynwb.validate should be updated such that all the loaded namespaces are validated together and you cannot specify a single namespace out of all the ones loaded to validate against. @oruebel @CodyCBakerPhD What do you think? This would be a major breaking change, but I think that is OK.