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Our list of external solutions is out of date and not as easy to maintain. Remove for now.
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README.md

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If you're using GPUs, or want to use Singularity instead, see
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[Quick Start](docs/deepvariant-quick-start.md) for more details or see all the
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[setup options](#deepvariant_setup) available including solutions on external
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platforms.
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[setup options](#deepvariant_setup) available.
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For more information, also see:
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[Accurate, scalable cohort variant calls using DeepVariant and GLnexus.
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_Bioinformatics_ (2021).](https://doi.org/10.1093/bioinformatics/btaa1081)<br/>
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Taedong Yun, Helen Li, Pi-Chuan Chang, Michael F. Lin, Andrew Carroll, and Cory Y.
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McLean.<br/>
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Taedong Yun, Helen Li, Pi-Chuan Chang, Michael F. Lin, Andrew Carroll, and Cory
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Y. McLean.<br/>
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doi: https://doi.org/10.1093/bioinformatics/btaa1081
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## Why Use DeepVariant?
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30x whole genome and $0.21 for whole exome (not considering preemption).
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* **Speed** - See [metrics](docs/metrics.md) for the runtime of all supported
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datatypes on a 64-core CPU-only machine</sup>. Multiple options for
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acceleration exist, taking the WGS pipeline to as fast as 40 minutes
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(see [external solutions](#external-solutions)).
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acceleration exist.
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* **Usage options** - DeepVariant can be run via Docker or binaries, using
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both on-premise hardware or in the cloud, with support for hardware
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accelerators like GPUs and TPUs.
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[Build from source](docs/deepvariant-build-test.md) | DeepVariant comes with scripts to build it on Ubuntu 20.04. To build and run on other Unix-based systems, you will need to modify these scripts.
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Prebuilt Binaries | Available at [`gs://deepvariant/`](https://console.cloud.google.com/storage/browser/deepvariant). These are compiled to use SSE4 and AVX instructions, so you will need a CPU (such as Intel Sandy Bridge) that supports them. You can check the `/proc/cpuinfo` file on your computer, which lists these features under "flags".
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### External Solutions
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The following pipelines are not created or maintained by the
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[Genomics team in Google Health](https://health.google/health-research/).
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Please contact the relevant teams if you have any questions or concerns.
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Name | Description
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:----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------: | -----------
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[Running DeepVariant on Google Cloud Platform](https://cloud.google.com/genomics/docs/tutorials/deepvariant) | Docker-based pipelines optimized for cost and speed. Code can be found [here](https://github.com/googlegenomics/gcp-deepvariant-runner).
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[DeepVariant-on-spark from ATGENOMIX](https://github.com/atgenomix/deepvariant-on-spark) | A germline short variant calling pipeline that runs DeepVariant on Apache Spark at scale with support for multi-GPU clusters (e.g. NVIDIA DGX-1).
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[NVIDIA Clara Parabricks](https://www.nvidia.com/en-us/docs/parabricks/variant-callers/deepvariant/) | An accelerated DeepVariant pipeline with multi-GPU support that runs our WGS pipeline in just 40 minutes, at a cost of $2-$3 per sample. This provides a 7.5x speedup over a 64-core CPU-only machine at lower cost.
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[DNAnexus DeepVariant App](https://platform.dnanexus.com/app/deepvariant_germline) | Offers parallelized execution with a GUI interface (requires platform account).
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[Nextflow Pipeline](https://github.com/nf-core/deepvariant) | Offers parallel processing of multiple BAMs and Docker support.
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[DNAstack Pipeline](https://app.dnastack.com/auth/realms/DNAstack/protocol/openid-connect/auth?client_id=dnastack-client&redirect_uri=https%3A%2F%2Fapp.dnastack.com%2F%3Fredirect_fragment%3D%252Forg%252F473079%252Fproj%252F473096%252Fapp%252Fworkflow%252F425685%252Frun&state=42231553-9fbc-4d71-a10e-d6ce42415c01&nonce=daf2568d-4fe7-48e2-ab60-858937244a87&response_mode=query&response_type=code&scope=openid) | Cost-optimized DeepVariant pipeline (requires platform account).
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## Contribution Guidelines
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Please [open a pull request](https://github.com/google/deepvariant/compare) if

docs/deeptrio-quick-start.md

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that you can use to build your own Docker image. You can read the [docker build]
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documentation on how to build.
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For production use cases on larger datasets, we recommend looking into the
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[External Solutions] section.
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## Get Docker image, models, and test data
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### Get Docker image
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[BWA]: https://academic.oup.com/bioinformatics/article/25/14/1754/225615/Fast-and-accurate-short-read-alignment-with
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[docker build]: https://docs.docker.com/engine/reference/commandline/build/
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[Dockerfile]: https://github.com/google/deepvariant/blob/r1.3/Dockerfile.deeptrio
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[External Solutions]: https://github.com/google/deepvariant#external-solutions
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[FASTA]: https://en.wikipedia.org/wiki/FASTA_format
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[VCF]: https://samtools.github.io/hts-specs/VCFv4.3.pdf
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[run_deeptrio.py]: ../scripts/run_deeptrio.py

docs/deepvariant-details.md

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We report runtime in our case studies documentation. In order to make sure the
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results we report are reproducible without too much variation, we provide the
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commands we used here to show you what kind of machines we ran the case studies
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on. This is NOT the fastest or cheapest configuration. For more scalable
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execution of DeepVariant see the [External Solutions] section.
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on. This is NOT the fastest or cheapest configuration.
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### Command for a CPU-only machine on Google Cloud Platform.
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docs/deepvariant-quick-start.md

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[Dockerfile] that you can use to build your own Docker image. You can read the
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[docker build] documentation on how to build.
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For production use cases on larger scale of dataset, we recommend looking into
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the [External Solutions] section.
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## Get Docker image, models, and test data
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### Get Docker image
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[BWA]: https://academic.oup.com/bioinformatics/article/25/14/1754/225615/Fast-and-accurate-short-read-alignment-with
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[docker build]: https://docs.docker.com/engine/reference/commandline/build/
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[Dockerfile]: https://github.com/google/deepvariant/blob/r1.3/Dockerfile
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[External Solutions]: https://github.com/google/deepvariant#external-solutions
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[FASTA]: https://en.wikipedia.org/wiki/FASTA_format
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[Quick Start in r0.7]: https://github.com/google/deepvariant/blob/r0.7/docs/deepvariant-quick-start.md
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[VCF]: https://samtools.github.io/hts-specs/VCFv4.3.pdf

docs/metrics-deeptrio.md

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For simplicity and consistency, we report runtime with a
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[CPU instance with 64 CPUs](deepvariant-details.md#command-for-a-cpu-only-machine-on-google-cloud-platform)
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For bigger datasets (WGS and PACBIO), we used bigger disk size (900G).
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This is NOT the fastest or cheapest configuration. For more scalable execution,
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see the [External Solutions] section.
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This is NOT the fastest or cheapest configuration.
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Use `gcloud compute ssh` to log in to the newly created instance.
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The accuracy metrics come from the hap.py summary.csv output file.
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The runs are deterministic so all 5 runs produced the same output.
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[External Solutions]: https://github.com/google/deepvariant#external-solutions
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[CPU instance with 64 CPUs]: deepvariant-details.md#command-for-a-cpu-only-machine-on-google-cloud-platform

docs/metrics.md

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For simplicity and consistency, we report runtime with a
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[CPU instance with 64 CPUs](deepvariant-details.md#command-for-a-cpu-only-machine-on-google-cloud-platform)
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This is NOT the fastest or cheapest configuration. For more scalable execution
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of DeepVariant see the [External Solutions] section.
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This is NOT the fastest or cheapest configuration.
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Use `gcloud compute ssh` to log in to the newly created instance.
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The accuracy metrics come from the hap.py summary.csv output file.
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The runs are deterministic so all 5 runs produced the same output.
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[External Solutions]: https://github.com/google/deepvariant#external-solutions
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[CPU instance with 64 CPUs]: deepvariant-details.md#command-for-a-cpu-only-machine-on-google-cloud-platform

docs/trio-merge-case-study.md

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A few things to note before we start:
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* If you are looking for ways to run DeepVariant in larger batches, please
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refer to the
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[third party solutions](https://github.com/google/deepvariant#external-solutions)
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section.
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* It is recommended to use BAM files with original quality scores. In the case
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that BAM files went through recalibration, optional DV flags can be used in
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order to use original scores: `--parse_sam_aux_fields`,

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