diff --git a/test/extended/prometheus/prometheus.go b/test/extended/prometheus/prometheus.go
index c146b85442b2..6772d96d8982 100644
--- a/test/extended/prometheus/prometheus.go
+++ b/test/extended/prometheus/prometheus.go
@@ -5,6 +5,8 @@ import (
 	"context"
 	"encoding/json"
 	"fmt"
+	"net/http"
+	"os"
 	"regexp"
 	"strings"
 	"time"
@@ -49,6 +51,101 @@ type TelemeterClientConfig struct {
 	Enabled *bool `json:"enabled"`
 }
 
+// Set $MONITORING_AUTH_TEST_NAMESPACE to focus on the targets from a single namespace
+var monitoringAuthTestNamespace = os.Getenv("MONITORING_AUTH_TEST_NAMESPACE")
+
+var _ = g.Describe("[sig-instrumentation][Late] Platform Prometheus targets", func() {
+	defer g.GinkgoRecover()
+	var (
+		oc                         = exutil.NewCLIWithPodSecurityLevel("prometheus", admissionapi.LevelBaseline)
+		prometheusURL, bearerToken string
+
+		// TODO: remove the namespace when the bug is fixed.
+		namespacesToSkip = sets.New[string]("openshift-marketplace", // https://issues.redhat.com/browse/OCPBUGS-59763
+			"openshift-image-registry",               // https://issues.redhat.com/browse/OCPBUGS-59767
+			"openshift-operator-lifecycle-manager",   // https://issues.redhat.com/browse/OCPBUGS-59768
+			"openshift-cluster-samples-operator",     // https://issues.redhat.com/browse/OCPBUGS-59769
+			"openshift-cluster-version",              // https://issues.redhat.com/browse/OCPBUGS-57585
+			"openshift-cluster-csi-drivers",          // https://issues.redhat.com/browse/OCPBUGS-60159
+			"openshift-cluster-node-tuning-operator", // https://issues.redhat.com/browse/OCPBUGS-60258
+			"openshift-etcd",                         // https://issues.redhat.com/browse/OCPBUGS-60263
+		)
+	)
+
+	g.BeforeEach(func(ctx g.SpecContext) {
+		var err error
+
+		kubeClient, err := kubernetes.NewForConfig(oc.AdminConfig())
+		o.Expect(err).NotTo(o.HaveOccurred())
+		nsExist, err := exutil.IsNamespaceExist(kubeClient, "openshift-monitoring")
+		o.Expect(err).NotTo(o.HaveOccurred())
+		if !nsExist {
+			g.Skip("openshift-monitoring namespace does not exist, skipping")
+		}
+
+		prometheusURL, err = helper.PrometheusRouteURL(ctx, oc)
+		o.Expect(err).NotTo(o.HaveOccurred(), "Get public url of prometheus")
+		bearerToken, err = helper.RequestPrometheusServiceAccountAPIToken(ctx, oc)
+		o.Expect(err).NotTo(o.HaveOccurred(), "Request prometheus service account API token")
+
+		if namespacesToSkip.Has(monitoringAuthTestNamespace) {
+			e2e.Logf("The namespace %s is not skipped because $MONITORING_AUTH_TEST_NAMESPACE is set to it", monitoringAuthTestNamespace)
+			namespacesToSkip.Delete(monitoringAuthTestNamespace)
+		}
+	})
+
+	g.It("should not be accessible without auth [Serial]", func() {
+		var errs []error
+
+		g.By("checking that targets reject the requests with 401 or 403")
+		execPod := exutil.CreateExecPodOrFail(oc.AdminKubeClient(), oc.Namespace(), "execpod-targets-authorization")
+		defer func() {
+			err := oc.AdminKubeClient().CoreV1().Pods(execPod.Namespace).Delete(context.Background(), execPod.Name, *metav1.NewDeleteOptions(1))
+			o.Expect(err).NotTo(o.HaveOccurred(), fmt.Sprintf("Delete pod %s/%s", execPod.Namespace, execPod.Name))
+		}()
+
+		contents, err := helper.GetURLWithToken(helper.MustJoinUrlPath(prometheusURL, "api/v1/targets"), bearerToken)
+		o.Expect(err).NotTo(o.HaveOccurred())
+
+		targets := &prometheusTargets{}
+		err = json.Unmarshal([]byte(contents), targets)
+		o.Expect(err).NotTo(o.HaveOccurred())
+		o.Expect(len(targets.Data.ActiveTargets)).Should(o.BeNumerically(">=", 5))
+
+		expected := sets.New[int](http.StatusUnauthorized, http.StatusForbidden)
+		for _, target := range targets.Data.ActiveTargets {
+			ns := target.Labels["namespace"]
+			o.Expect(ns).NotTo(o.BeEmpty())
+			if monitoringAuthTestNamespace != "" && ns != monitoringAuthTestNamespace {
+				continue
+			}
+			pod := target.Labels["pod"]
+			e2e.Logf("Checking via pod exec status code from the scrape url %s for pod %s/%s without authorization (skip=%t)", target.ScrapeUrl, ns, pod, namespacesToSkip.Has(ns))
+			err := wait.PollUntilContextTimeout(context.Background(), 10*time.Second, time.Minute, true, func(context.Context) (bool, error) {
+				statusCode, execError := helper.URLStatusCodeExecViaPod(execPod.Namespace, execPod.Name, target.ScrapeUrl)
+				e2e.Logf("The scrape url %s for pod %s/%s without authorization returned %d, %v (skip=%t)", target.ScrapeUrl, ns, pod, statusCode, execError, namespacesToSkip.Has(ns))
+				if expected.Has(statusCode) {
+					return true, nil
+				}
+				// retry on those cases
+				if execError != nil ||
+					statusCode/100 == 5 ||
+					statusCode == http.StatusRequestTimeout ||
+					statusCode == http.StatusTooManyRequests {
+					return false, nil
+				}
+				return false, fmt.Errorf("expecting status code %v but returned %d", expected.UnsortedList(), statusCode)
+			})
+			if err != nil && !namespacesToSkip.Has(ns) {
+				errs = append(errs, fmt.Errorf("the scrape url %s for pod %s/%s is accessible without authorization: %w", target.ScrapeUrl, ns, pod, err))
+			}
+		}
+
+		o.Expect(errs).To(o.BeEmpty())
+	})
+
+})
+
 var _ = g.Describe("[sig-instrumentation][Late] OpenShift alerting rules [apigroup:image.openshift.io]", func() {
 	defer g.GinkgoRecover()
 
diff --git a/test/extended/util/annotate/generated/zz_generated.annotations.go b/test/extended/util/annotate/generated/zz_generated.annotations.go
index 9db297270b06..da7a53f442a9 100644
--- a/test/extended/util/annotate/generated/zz_generated.annotations.go
+++ b/test/extended/util/annotate/generated/zz_generated.annotations.go
@@ -1405,6 +1405,8 @@ var Annotations = map[string]string{
 
 	"[sig-instrumentation][Late] OpenShift alerting rules [apigroup:image.openshift.io] should link to an HTTP(S) location if the runbook_url annotation is defined": " [Suite:openshift/conformance/parallel]",
 
+	"[sig-instrumentation][Late] Platform Prometheus targets should not be accessible without auth [Serial]": " [Suite:openshift/conformance/serial]",
+
 	"[sig-instrumentation][OCPFeatureGate:MetricsCollectionProfiles][Serial] The collection profiles feature-set in a heterogeneous environment, should expose information about the applied collection profile using meta-metrics": " [Suite:openshift/conformance/serial]",
 
 	"[sig-instrumentation][OCPFeatureGate:MetricsCollectionProfiles][Serial] The collection profiles feature-set in a heterogeneous environment, should have at least one implementation for each collection profile": " [Suite:openshift/conformance/serial]",
diff --git a/test/extended/util/prometheus/helpers.go b/test/extended/util/prometheus/helpers.go
index 6a266eafb7cd..1dc11eea28d3 100644
--- a/test/extended/util/prometheus/helpers.go
+++ b/test/extended/util/prometheus/helpers.go
@@ -299,6 +299,21 @@ func ExpectURLStatusCodeExecViaPod(ns, execPodName, url string, statusCodes ...i
 	return fmt.Errorf("last response from server was not in %v: %s", statusCodes, output)
 }
 
+// URLStatusCodeExecViaPod attempts connection to url via exec pod and returns the status code
+// or an error if any errors happens during the process.
+func URLStatusCodeExecViaPod(ns, name, url string) (int, error) {
+	cmd := fmt.Sprintf("curl -k -s -o /dev/null -w '%%{http_code}' %q", url)
+	output, err := e2eoutput.RunHostCmd(ns, name, cmd)
+	if err != nil {
+		return 0, fmt.Errorf("host command failed: %v\n%s", err, output)
+	}
+	ret, err := strconv.Atoi(output)
+	if err != nil {
+		return 0, fmt.Errorf("unable to parse status code out of the command's ouput: %v\n%s", err, output)
+	}
+	return ret, nil
+}
+
 // ExpectPrometheusEndpoint attempts to connect to the metrics endpoint with
 // delayed retries upon failure.
 func ExpectPrometheusEndpoint(url string) {