Hello,
I'm using BBmerge to determine which adapter sequences on my short reads (as suggested on http://seqanswers.com/forums/showthread.php?t=63537). I was wondering if you could help with an error I'm getting. When I run this line from my SLURM script:
scripts="/gpfs/scratch/sjfleck/modulefiles/BBMap/sh"
fastq="/gpfs/scratch/sjfleck/MaSuRCA/my_species"
$scripts/bbmerge.sh $fastq/in1=forward_reads_1.fq.gz $fastq/in2=reverse_reads_2.fq.gz outa=my_species_adapters.fa
Here is the error file I get (I'm separating some lines for clarity):
java -Djava.library.path=/gpfs/scratch/sjfleck/modulefiles/BBMap/sh/jni/ -ea -Xmx1000m -cp /gpfs/scratch/sjfleck/modulefiles/BBMap/sh/current/
in1=forward_reads_1.fq.gz
in2=reverse_reads_2.fq.gz
outa=Ping_moct_adapters.fa
Error: Could not find or load main class jgi.BBMerge
There are a few things I'm immediately noticing:
- there is no file named "jgi.BBMerge" in "/gpfs/scratch/sjfleck/modulefiles/BBMap/sh/current/"
- There is a folder called "jgi" in this directory, but no files.
- within the "jgi" directory, the closest thing to "jgi.BBMerge" is "BBMerge.java" and "BBMergeOverlapper.java"
- " jgi.BBMerge" starts with a space in the script. Is this by design?
Any help you could offer would be greatly appreciated. Thank you,
Steve
Hello,
I'm using BBmerge to determine which adapter sequences on my short reads (as suggested on http://seqanswers.com/forums/showthread.php?t=63537). I was wondering if you could help with an error I'm getting. When I run this line from my SLURM script:
scripts="/gpfs/scratch/sjfleck/modulefiles/BBMap/sh"
fastq="/gpfs/scratch/sjfleck/MaSuRCA/my_species"
$scripts/bbmerge.sh $fastq/in1=forward_reads_1.fq.gz $fastq/in2=reverse_reads_2.fq.gz outa=my_species_adapters.fa
Here is the error file I get (I'm separating some lines for clarity):
java -Djava.library.path=/gpfs/scratch/sjfleck/modulefiles/BBMap/sh/jni/ -ea -Xmx1000m -cp /gpfs/scratch/sjfleck/modulefiles/BBMap/sh/current/
in1=forward_reads_1.fq.gz
in2=reverse_reads_2.fq.gz
outa=Ping_moct_adapters.fa
Error: Could not find or load main class jgi.BBMerge
There are a few things I'm immediately noticing:
Any help you could offer would be greatly appreciated. Thank you,
Steve