new user problem

[asantos01]

Hi, i am analyzing a MinION sequencer dataset that contains 16S gene sequences (1500 pb each) but i am having the next issue with the hmmufotu command :

MSA loaded
HMM profile read
CSFM-index loaded
Phylogenetic tree loaded
Determining read strand by alignment cost ...
terminate called after throwing an instance of 'std::invalid_argument'
what(): Your sequence contains invalid alphabet charactersCAGACGACTGCAAACGGAATCGGAGTCACGCACC
Aborted (core dumped)

i have tried with another 16S small dataset from MinION and it worked well....

can someone help me please?

Thanks!

[e00011027]

Hi, It may be caused by unprintable but illegal characters in your input file. Characters such as null ('\0'), Mac OS new line ('\r') may not be displayed but in the file. Try to open your input with vim or other text editors to find invalid characters. Best Qi

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On Wed, Sep 19, 2018 at 1:46 PM asantos01 ***@***.***> wrote: Hi, i am analyzing a MinION sequencer dataset that contains 16S gene sequences (1500 pb each) but i am having the next issue with the hmmufotu command : MSA loaded HMM profile read CSFM-index loaded Phylogenetic tree loaded Determining read strand by alignment cost ... terminate called after throwing an instance of 'std::invalid_argument' what(): Your sequence contains invalid alphabet charactersCAGACGACTGCAAACGGAATCGGAGTCACGCACC Aborted (core dumped) i have tried with another 16S small dataset from MinION and it worked well.... can someone help me please? Thanks! — You are receiving this because you are subscribed to this thread. Reply to this email directly, view it on GitHub <#1>, or mute the thread <https://github.com/notifications/unsubscribe-auth/AG-ecTr1xUIhY1qYCiPZ-8WlxzEznbteks5ucoLwgaJpZM4Wwpxw> .