diff --git a/src/lightning/pytorch/loggers/mlflow.py b/src/lightning/pytorch/loggers/mlflow.py
index ff9b2b0d7e542..a904a79861e3a 100644
--- a/src/lightning/pytorch/loggers/mlflow.py
+++ b/src/lightning/pytorch/loggers/mlflow.py
@@ -300,7 +300,18 @@ def save_dir(self) -> Optional[str]:
 
         """
         if self._tracking_uri.startswith(LOCAL_FILE_URI_PREFIX):
-            return self._tracking_uri[len(LOCAL_FILE_URI_PREFIX) :]
+            # Handle both proper file URIs (file:///path) and legacy format (file:/path)
+            uri_without_prefix = self._tracking_uri[len(LOCAL_FILE_URI_PREFIX) :]
+
+            # If it starts with ///, it's a proper file URI, use urlparse
+            if uri_without_prefix.startswith("///"):
+                from urllib.parse import urlparse
+                from urllib.request import url2pathname
+
+                parsed_uri = urlparse(self._tracking_uri)
+                return url2pathname(parsed_uri.path)
+            # Legacy format: file:/path or file:./path - return as-is
+            return uri_without_prefix
         return None
 
     @property
diff --git a/tests/tests_pytorch/loggers/test_mlflow.py b/tests/tests_pytorch/loggers/test_mlflow.py
index c7f9dbe1fe2c6..9de0ad23a93cf 100644
--- a/tests/tests_pytorch/loggers/test_mlflow.py
+++ b/tests/tests_pytorch/loggers/test_mlflow.py
@@ -427,3 +427,43 @@ def test_set_tracking_uri(mlflow_mock):
     mlflow_mock.set_tracking_uri.assert_not_called()
     _ = logger.experiment
     mlflow_mock.set_tracking_uri.assert_called_with("the_tracking_uri")
+
+
+@mock.patch("lightning.pytorch.loggers.mlflow._get_resolve_tags", Mock())
+def test_mlflow_logger_save_dir_file_uri_handling(mlflow_mock):
+    """Test that save_dir correctly handles file URIs, especially on Windows."""
+    import platform
+
+    # Test proper Windows-style absolute file URI (the main fix)
+    logger_win = MLFlowLogger(tracking_uri="file:///C:/Dev/example/mlruns")
+    result_win = logger_win.save_dir
+    expected_win = "C:\\Dev\\example\\mlruns" if platform.system() == "Windows" else "/C:/Dev/example/mlruns"
+    assert result_win == expected_win
+
+    # Test proper Unix-style absolute file URI
+    logger_unix = MLFlowLogger(tracking_uri="file:///home/user/mlruns")
+    result_unix = logger_unix.save_dir
+    expected_unix = "\\home\\user\\mlruns" if platform.system() == "Windows" else "/home/user/mlruns"
+    assert result_unix == expected_unix
+
+    # Test proper file URI with special characters and spaces
+    logger_special = MLFlowLogger(tracking_uri="file:///path/with%20spaces/mlruns")
+    result_special = logger_special.save_dir
+    expected_special = "\\path\\with spaces\\mlruns" if platform.system() == "Windows" else "/path/with spaces/mlruns"
+    assert result_special == expected_special
+
+    # Test legacy format used by constructor (file:/path - should return as-is)
+    logger_legacy = MLFlowLogger(tracking_uri="file:/tmp/mlruns")
+    result_legacy = logger_legacy.save_dir
+    expected_legacy = "/tmp/mlruns"
+    assert result_legacy == expected_legacy
+
+    # Test legacy relative format
+    logger_rel = MLFlowLogger(tracking_uri="file:./mlruns")
+    result_rel = logger_rel.save_dir
+    expected_rel = "./mlruns"
+    assert result_rel == expected_rel
+
+    # Test non-file URI (should return None)
+    logger_http = MLFlowLogger(tracking_uri="http://localhost:8080")
+    assert logger_http.save_dir is None