Skip to content

error in transcript quantification step #44

@sparthib

Description

@sparthib

Hi, I am getting this error in the final counts matrix generation step:

does anyone know how to circumvent this issue?

b'[bam_sort_core] merging from 9 files and 12 in-memory blocks...\n'
b''
### generate transcript count matrix 2023-12-08 17:21:24
Traceback (most recent call last):
  File "/users/sparthib/flames/python/bulk_long_pipeline.py", line 270, in <module>
    bulk_long_pipeline(args)
  File "/users/sparthib/flames/python/bulk_long_pipeline.py", line 235, in bulk_long_pipeline
    bc_tr_count_dict, bc_tr_badcov_count_dict, tr_kept = parse_realigned_bam(
  File "/users/sparthib/flames/python/count_tr.py", line 114, in parse_realigned_bam
    bc_dict = make_bc_dict(kwargs["bc_file"])
  File "/users/sparthib/flames/python/count_tr.py", line 57, in make_bc_dict
    with open(bc_anno) as f:
FileNotFoundError: [Errno 2] No such file or directory: ''

thanks!

Sowmya

Metadata

Metadata

Assignees

No one assigned

    Labels

    No labels
    No labels

    Projects

    No projects

    Milestone

    No milestone

    Relationships

    None yet

    Development

    No branches or pull requests

    Issue actions