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Separate sync as its own stream in SpikeGLXRawIO (#1683)
* spikeglx sync PR * improve message * forgot comma * order --------- Co-authored-by: Zach McKenzie <[email protected]>
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-9
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neo/rawio/spikeglxrawio.py

Lines changed: 49 additions & 8 deletions
Original file line numberDiff line numberDiff line change
@@ -53,6 +53,7 @@
5353
from pathlib import Path
5454
import os
5555
import re
56+
from warnings import warn
5657

5758
import numpy as np
5859

@@ -76,7 +77,9 @@ class SpikeGLXRawIO(BaseRawWithBufferApiIO):
7677
dirname: str, default: ''
7778
The spikeglx folder containing meta/bin files
7879
load_sync_channel: bool, default: False
79-
The last channel (SY0) of each stream is a fake channel used for synchronisation
80+
Can be used to load the synch stream as the last channel of the neural data.
81+
This option is deprecated and will be removed in version 0.15.
82+
From versions higher than 0.14.1 the sync channel is always loaded as a separate stream.
8083
load_channel_location: bool, default: False
8184
If True probeinterface is used to load the channel locations from the directory
8285
@@ -109,6 +112,12 @@ def __init__(self, dirname="", load_sync_channel=False, load_channel_location=Fa
109112
BaseRawWithBufferApiIO.__init__(self)
110113
self.dirname = dirname
111114
self.load_sync_channel = load_sync_channel
115+
if load_sync_channel:
116+
warn(
117+
"The load_sync_channel=True option is deprecated and will be removed in version 0.15 \n"
118+
"The sync channel is now loaded as a separate stream by default and should be accessed as such. ",
119+
DeprecationWarning, stacklevel=2
120+
)
112121
self.load_channel_location = load_channel_location
113122

114123
def _source_name(self):
@@ -152,6 +161,8 @@ def _parse_header(self):
152161
signal_buffers = []
153162
signal_streams = []
154163
signal_channels = []
164+
sync_stream_id_to_buffer_id = {}
165+
155166
for stream_name in stream_names:
156167
# take first segment
157168
info = self.signals_info_dict[0, stream_name]
@@ -168,6 +179,16 @@ def _parse_header(self):
168179
for local_chan in range(info["num_chan"]):
169180
chan_name = info["channel_names"][local_chan]
170181
chan_id = f"{stream_name}#{chan_name}"
182+
183+
# Sync channel
184+
if "nidq" not in stream_name and "SY0" in chan_name and not self.load_sync_channel and local_chan == info["num_chan"] - 1:
185+
# This is a sync channel and should be added as its own stream
186+
sync_stream_id = f"{stream_name}-SYNC"
187+
sync_stream_id_to_buffer_id[sync_stream_id] = buffer_id
188+
stream_id_for_chan = sync_stream_id
189+
else:
190+
stream_id_for_chan = stream_id
191+
171192
signal_channels.append(
172193
(
173194
chan_name,
@@ -177,25 +198,33 @@ def _parse_header(self):
177198
info["units"],
178199
info["channel_gains"][local_chan],
179200
info["channel_offsets"][local_chan],
180-
stream_id,
201+
stream_id_for_chan,
181202
buffer_id,
182203
)
183204
)
184205

185-
# all channel by dafult unless load_sync_channel=False
206+
# all channel by default unless load_sync_channel=False
186207
self._stream_buffer_slice[stream_id] = None
208+
187209
# check sync channel validity
188210
if "nidq" not in stream_name:
189211
if not self.load_sync_channel and info["has_sync_trace"]:
190-
# the last channel is remove from the stream but not from the buffer
191-
last_chan = signal_channels[-1]
192-
last_chan = last_chan[:-2] + ("", buffer_id)
193-
signal_channels = signal_channels[:-1] + [last_chan]
212+
# the last channel is removed from the stream but not from the buffer
194213
self._stream_buffer_slice[stream_id] = slice(0, -1)
214+
215+
# Add a buffer slice for the sync channel
216+
sync_stream_id = f"{stream_name}-SYNC"
217+
self._stream_buffer_slice[sync_stream_id] = slice(-1, None)
218+
195219
if self.load_sync_channel and not info["has_sync_trace"]:
196220
raise ValueError("SYNC channel is not present in the recording. " "Set load_sync_channel to False")
197221

198222
signal_buffers = np.array(signal_buffers, dtype=_signal_buffer_dtype)
223+
224+
# Add sync channels as their own streams
225+
for sync_stream_id, buffer_id in sync_stream_id_to_buffer_id.items():
226+
signal_streams.append((sync_stream_id, sync_stream_id, buffer_id))
227+
199228
signal_streams = np.array(signal_streams, dtype=_signal_stream_dtype)
200229
signal_channels = np.array(signal_channels, dtype=_signal_channel_dtype)
201230

@@ -237,6 +266,14 @@ def _parse_header(self):
237266
t_start = frame_start / sampling_frequency
238267

239268
self._t_starts[stream_name][seg_index] = t_start
269+
270+
# This need special logic because sync not present in stream_names
271+
if f"{stream_name}-SYNC" in signal_streams["name"]:
272+
sync_stream_name = f"{stream_name}-SYNC"
273+
if sync_stream_name not in self._t_starts:
274+
self._t_starts[sync_stream_name] = {}
275+
self._t_starts[sync_stream_name][seg_index] = t_start
276+
240277
t_stop = info["sample_length"] / info["sampling_rate"]
241278
self._t_stops[seg_index] = max(self._t_stops[seg_index], t_stop)
242279

@@ -265,7 +302,11 @@ def _parse_header(self):
265302
if self.load_channel_location:
266303
# need probeinterface to be installed
267304
import probeinterface
268-
305+
306+
# Skip for sync streams
307+
if "SYNC" in stream_name:
308+
continue
309+
269310
info = self.signals_info_dict[seg_index, stream_name]
270311
if "imroTbl" in info["meta"] and info["stream_kind"] == "ap":
271312
# only for ap channel

neo/test/rawiotest/test_spikeglxrawio.py

Lines changed: 25 additions & 1 deletion
Original file line numberDiff line numberDiff line change
@@ -55,7 +55,7 @@ def test_loading_only_one_probe_in_multi_probe_scenario(self):
5555
rawio = SpikeGLXRawIO(probe_folder_path)
5656
rawio.parse_header()
5757

58-
expected_stream_names = ["imec1.ap", "imec1.lf"]
58+
expected_stream_names = ["imec1.ap", "imec1.lf", "imec1.ap-SYNC", "imec1.lf-SYNC"]
5959
actual_stream_names = rawio.header["signal_streams"]["name"].tolist()
6060
assert (
6161
actual_stream_names == expected_stream_names
@@ -130,6 +130,30 @@ def test_nidq_digital_channel(self):
130130
atol = 0.001
131131
assert np.allclose(on_diff, 1, atol=atol)
132132

133+
def test_sync_channel_as_separate_stream(self):
134+
"""Test that sync channel is added as its own stream when load_sync_channel=False."""
135+
import warnings
136+
137+
# Test with load_sync_channel=False (default)
138+
rawio_no_sync = SpikeGLXRawIO(self.get_local_path("spikeglx/NP2_with_sync"), load_sync_channel=False)
139+
rawio_no_sync.parse_header()
140+
141+
# Get stream names
142+
stream_names = rawio_no_sync.header["signal_streams"]["name"].tolist()
143+
144+
# Check if there's a sync channel stream (should contain "SY0" or "SYNC" in the name)
145+
sync_streams = [name for name in stream_names if "SY0" in name or "SYNC" in name]
146+
assert len(sync_streams) > 0, "No sync channel stream found when load_sync_channel=False"
147+
148+
# Test deprecation warning when load_sync_channel=True
149+
with warnings.catch_warnings(record=True) as w:
150+
warnings.simplefilter("always")
151+
rawio_with_sync = SpikeGLXRawIO(self.get_local_path("spikeglx/NP2_with_sync"), load_sync_channel=True)
152+
153+
# Check if deprecation warning was raised
154+
assert any(issubclass(warning.category, DeprecationWarning) for warning in w), "No deprecation warning raised"
155+
assert any("will be removed in version 0.15" in str(warning.message) for warning in w), "Deprecation warning message is incorrect"
156+
133157
def test_t_start_reading(self):
134158
"""Test that t_start values are correctly read for all streams and segments."""
135159

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