diff --git a/.github/workflows/integration-tests-against-emulator-3.14.yml b/.github/workflows/integration-tests-against-emulator-3.14.yml
new file mode 100644
index 0000000000..f2865975f4
--- /dev/null
+++ b/.github/workflows/integration-tests-against-emulator-3.14.yml
@@ -0,0 +1,35 @@
+on:
+  push:
+    branches:
+      - main
+  pull_request:
+permissions:
+  contents: read
+  pull-requests: write
+name: Run Django Spanner integration tests against emulator 3.14
+jobs:
+  system-tests:
+    runs-on: ubuntu-latest
+
+    services:
+      emulator:
+        image: gcr.io/cloud-spanner-emulator/emulator:latest
+        ports:
+          - 9010:9010
+          - 9020:9020
+
+    steps:
+      - name: Checkout code
+        uses: actions/checkout@v4
+      - name: Set up Python 3.14
+        uses: actions/setup-python@v5
+        with:
+          python-version: "3.14"
+      - name: Install nox
+        run: python -m pip install nox
+      - name: Run nox
+        run: nox -s unit-3.14
+        env:
+          SPANNER_EMULATOR_HOST: localhost:9010
+          GOOGLE_CLOUD_PROJECT: emulator-test-project
+          GOOGLE_CLOUD_TESTS_CREATE_SPANNER_INSTANCE: true
diff --git a/.github/workflows/mockserver-tests.yml b/.github/workflows/mockserver-tests.yml
index 1cdc634fa1..1322a8d371 100644
--- a/.github/workflows/mockserver-tests.yml
+++ b/.github/workflows/mockserver-tests.yml
@@ -11,10 +11,10 @@ jobs:
     steps:
       - name: Checkout code
         uses: actions/checkout@v4
-      - name: Set up Python 3.12
+      - name: Set up Python 3.14
         uses: actions/setup-python@v5
         with:
-          python-version: "3.12"
+          python-version: "3.14"
       - name: Install nox
         run: python -m pip install nox
       - name: Run nox
diff --git a/noxfile.py b/noxfile.py
index cb438618f7..3d819b4799 100644
--- a/noxfile.py
+++ b/noxfile.py
@@ -24,10 +24,10 @@
     "setup.py",
 ]
 
-MOCKSERVER_TEST_PYTHON_VERSION = "3.12"
+MOCKSERVER_TEST_PYTHON_VERSION = "3.14"
 DEFAULT_PYTHON_VERSION = "3.8"
 SYSTEM_TEST_PYTHON_VERSIONS = ["3.8"]
-UNIT_TEST_PYTHON_VERSIONS = ["3.8", "3.9", "3.10"]
+UNIT_TEST_PYTHON_VERSIONS = ["3.8", "3.9", "3.10", "3.14"]
 
 CURRENT_DIRECTORY = pathlib.Path(__file__).parent.absolute()
 
diff --git a/tests/mockserver_tests/test_basics.py b/tests/mockserver_tests/test_basics.py
index 489ebf3cbf..a965486a48 100644
--- a/tests/mockserver_tests/test_basics.py
+++ b/tests/mockserver_tests/test_basics.py
@@ -13,6 +13,7 @@
 # limitations under the License.
 from google.cloud.spanner_v1 import (
     BatchCreateSessionsRequest,
+    CreateSessionRequest,
     ExecuteSqlRequest,
     CommitRequest,
 )
@@ -36,9 +37,10 @@ def verify_select1(self, results):
             self.assertEqual(row[0], 1)
         self.assertEqual(len(result_list), 1)
         requests = self.spanner_service.requests
-        self.assertEqual(len(requests), 2)
+        self.assertEqual(len(requests), 3)
         self.assertIsInstance(requests[0], BatchCreateSessionsRequest)
-        self.assertIsInstance(requests[1], ExecuteSqlRequest)
+        self.assertIsInstance(requests[1], CreateSessionRequest)
+        self.assertIsInstance(requests[2], ExecuteSqlRequest)
 
     def test_select1(self):
         add_select1_result()
@@ -59,9 +61,10 @@ def test_django_select_singer(self):
         singers = Singer.objects.all()
         self.assertEqual(len(singers), 2)
         requests = self.spanner_service.requests
-        self.assertEqual(len(requests), 2)
+        self.assertEqual(len(requests), 3)
         self.assertIsInstance(requests[0], BatchCreateSessionsRequest)
-        self.assertIsInstance(requests[1], ExecuteSqlRequest)
+        self.assertIsInstance(requests[1], CreateSessionRequest)
+        self.assertIsInstance(requests[2], ExecuteSqlRequest)
 
     def test_django_select_singer_using_other_db(self):
         add_singer_query_result(
@@ -70,9 +73,10 @@ def test_django_select_singer_using_other_db(self):
         singers = Singer.objects.using("secondary").all()
         self.assertEqual(len(singers), 2)
         requests = self.spanner_service.requests
-        self.assertEqual(len(requests), 2)
+        self.assertEqual(len(requests), 3)
         self.assertIsInstance(requests[0], BatchCreateSessionsRequest)
-        self.assertIsInstance(requests[1], ExecuteSqlRequest)
+        self.assertIsInstance(requests[1], CreateSessionRequest)
+        self.assertIsInstance(requests[2], ExecuteSqlRequest)
 
     def test_insert_singer(self):
         add_update_count(
@@ -84,15 +88,16 @@ def test_insert_singer(self):
         singer = Singer(first_name="test", last_name="test")
         singer.save()
         requests = self.spanner_service.requests
-        self.assertEqual(len(requests), 3)
+        self.assertEqual(len(requests), 4)
         self.assertIsInstance(requests[0], BatchCreateSessionsRequest)
-        self.assertIsInstance(requests[1], ExecuteSqlRequest)
-        self.assertIsInstance(requests[2], CommitRequest)
+        self.assertIsInstance(requests[1], CreateSessionRequest)
+        self.assertIsInstance(requests[2], ExecuteSqlRequest)
+        self.assertIsInstance(requests[3], CommitRequest)
         # The ExecuteSqlRequest should have 3 parameters:
         # 1. first_name
         # 2. last_name
         # 3. client-side auto-generated primary key
-        self.assertEqual(len(requests[1].params), 3)
+        self.assertEqual(len(requests[2].params), 3)
 
     def test_insert_singer_with_disabled_random_primary_key(self):
         for db, config in DATABASES.items():
@@ -115,15 +120,16 @@ class LocalSinger(models.Model):
             singer = LocalSinger(first_name="test", last_name="test")
             singer.save()
             requests = self.spanner_service.requests
-            self.assertEqual(len(requests), 3)
+            self.assertEqual(len(requests), 4)
             self.assertIsInstance(requests[0], BatchCreateSessionsRequest)
-            self.assertIsInstance(requests[1], ExecuteSqlRequest)
-            self.assertIsInstance(requests[2], CommitRequest)
+            self.assertIsInstance(requests[1], CreateSessionRequest)
+            self.assertIsInstance(requests[2], ExecuteSqlRequest)
+            self.assertIsInstance(requests[3], CommitRequest)
             # The ExecuteSqlRequest should have 2 parameters:
             # 1. first_name
             # 2. last_name
             # There should be no client-side auto-generated primary key.
-            self.assertEqual(len(requests[1].params), 2)
+            self.assertEqual(len(requests[2].params), 2)
         finally:
             for db, config in DATABASES.items():
                 if config["ENGINE"] == "django_spanner":