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Hi I hope you are still checking here :)
java -jar SimSeq.jar -1 100 -2 100 --error SimSeq-master/examples/hiseq_mito_default_bwa_mapping_mq10_1.txt --error2 SimSeq-master/examples/hiseq_mito_default_bwa_mapping_mq10_2.txt --insert_size 2000 --insert_stdev 200 --mate_pair --mate_frag 500 --mate_frag_stdev 50 --mate_pulldown_error_p 0.3 --reference ../S/F/X.fasta --duplicate_probability 0.01 --out X.sam
This code generated a sam file as expected but when I try converting sam to bam file it gives error No SQ lines present in the header. I checked manually and SAM file doesn't contain SQ lines as it is said.
Do you have any ideas?
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