Use of dmrseq with only one sample per condition/using Pacbio data

[epigenomepostdoc]

Hello! I am trying to explore different software for identifying DMRs. While my dataset is Pacbio hifi => pbcpg to ger perCpG methylation calls, I am wondering if dmrseq can still be used using the pbcpg output. Also, I do not have any replicates since my 8 samples are being compared in a pairwise manner (no controls/case).

I appreciate your feedback and suggestions. Thank you very much.

[kdkorthauer]

Hi! I'm afraid that since dmrseq is designed to perform inference, it requires replicates.

[epigenomepostdoc]

Thank you very much for your response!