@@ -62,7 +62,7 @@ Two heuristics are applied for increasing specificity:
6262
6363### Running Secphase in marker mode
6464
65- To run Secphase it is recommended to use the docker image ` mobinasri/secphase:v0.4.1 ` .
65+ To run Secphase it is recommended to use the docker image ` mobinasri/secphase:v0.4.2 ` .
6666
6767Here are the parameters ` secphase ` can accept:
6868```
@@ -108,15 +108,15 @@ samtools sort -n -@8 ${INPUT_DIR}/${BAM_PREFIX}.bam > ${INPUT_DIR}/${BAM_PREFIX}
108108## Create secphase index
109109docker run \
110110 -v ${INPUT_DIR}:${INPUT_DIR} \
111- mobinasri/secphase:v0.4.1 \
111+ mobinasri/secphase:v0.4.2 \
112112 secphase_index \
113113 -i ${INPUT_DIR}/${BAM_PREFIX}.sorted.bam
114114
115115## Run Secphase for HiFi alignments
116116docker run \
117117 -v ${INPUT_DIR}:${INPUT_DIR} \
118118 -v ${OUTPUT_DIR}:${OUTPUT_DIR}
119- mobinasri/secphase:v0.4.1 \
119+ mobinasri/secphase:v0.4.2 \
120120 secphase --hifi \
121121 -i ${INPUT_DIR}/${BAM_PREFIX}.sorted.bam \
122122 -f ${INPUT_DIR}/${FASTA_PREFIX}.fa \
@@ -128,7 +128,7 @@ docker run \
128128docker run \
129129 -v ${INPUT_DIR}:${INPUT_DIR} \
130130 -v ${OUTPUT_DIR}:${OUTPUT_DIR}
131- mobinasri/secphase:v0.4.1 \
131+ mobinasri/secphase:v0.4.2 \
132132 secphase --ont \
133133 -i ${INPUT_DIR}/${BAM_PREFIX}.sorted.bam \
134134 -f ${INPUT_DIR}/${FASTA_PREFIX}.fa \
@@ -162,7 +162,7 @@ For producing a phased vcf file one approach can be aligning all HiFi reads to e
162162docker run \
163163 -v ${INPUT_DIR}:${INPUT_DIR} \
164164 -v ${OUTPUT_DIR}:${OUTPUT_DIR}
165- mobinasri/secphase:v0.4.1 \
165+ mobinasri/secphase:v0.4.2 \
166166 secphase --ont \
167167 -i ${INPUT_DIR}/${BAM_PREFIX}.sorted.bam \
168168 -f ${INPUT_DIR}/${FASTA_PREFIX}.fa \
@@ -175,7 +175,7 @@ docker run \
175175### Correcting bam file with secphase output
176176
177177To swap the pri/sec tags of the reads reported in ` *.out.log ` and produce a modified bam file you can run the program ` correct_bam ` .
178- Again it is recommended to run it using the docker image ` mobinasri/secphase:v0.4.1 ` .
178+ Again it is recommended to run it using the docker image ` mobinasri/secphase:v0.4.2 ` .
179179
180180Here are the parameters ` correct_bam ` can accept:
181181```
@@ -214,7 +214,7 @@ To produce the modified bam file: (Here the input bam file can be sorted by refe
214214docker run \
215215 -v ${INPUT_DIR}:${INPUT_DIR} \
216216 -v ${OUTPUT_DIR}:${OUTPUT_DIR} \
217- mobinasri/secphase:v0.4.1 \
217+ mobinasri/secphase:v0.4.2 \
218218 correct_bam \
219219 -i ${INPUT_DIR}/${BAM_PREFIX}.bam \
220220 -P ${OUTPUT_DIR}/${SAMPLE_NAME}.out.log \
0 commit comments