Skip to content

Problem with "run_analysis_gated" #15

@CeliaJuarillo

Description

@CeliaJuarillo

Hi,
I'm trying to run "run_analysis_gated" but I keep encountering the same error:
"Markers used for SCAFFoLD: CD45RA, CD8, CCR7, CD3, CD4, CD25, CD56, CD127, HLADR, CD38"
[1] "Using as reference Panel_Pep_Javier T+NK 230124-BL_01 CNT-ALL BL-1 CONTROL_Unmixed.fcs.clustered.txt"
[1] "Downsampling to 1000 events"
[1] "Processing /home/cjuarez/Escritorio/spectral/cyclone_new/outputs/scaffold/Panel_Pep_Javier T+NK 230124-BL_01 CNT-ALL BL-1 CONTROL_Unmixed.fcs.clustered.txt"
[1] "Running with Edge weight: 4.000000"
Error in [.data.frame(tab, , col.names) : undefined columns selected
Calls: ... process_data -> build_graph -> as.matrix -> [ -> [.data.frame
Además: Warning messages:
1: replacing previous import ‘flowCore::normalize’ by ‘igraph::normalize’ when loading ‘scaffold’
2: replacing previous import ‘plyr::rename’ by ‘reshape::rename’ when loading ‘scaffold’
3: replacing previous import ‘plyr::round_any’ by ‘reshape::round_any’ when loading ‘scaffold’
4: '.local' is deprecated.
Use 'keyword' instead.
See help("Deprecated")

I tried using it with less and different markers in case it has something to do with some problem with the values for one of the columns of markers, but then I obtain this error:
Error in apply(tab[, col.names], 1, function(x) { :
dim(X) must have a positive length
Además: Warning message:
'.local' is deprecated.
Use 'keyword' instead.
See help("Deprecated")

I don't know what it's happening my code is this:
scaffold:::run_analysis_gated("/home/cjuarez/Escritorio/spectral/cyclone_new/outputs/scaffold", #scaffold_dir,
"Panel_Pep_Javier T+NK 230124-BL_01 CNT-ALL BL-1 CONTROL_Unmixed.fcs.clustered.txt", #ref_dataset_file
c("CD8", "CCR7", "CD3", "CD4", "CD45RA", "CD25", "CD56", "CD127", "HLADR", "CD38"), #marker_metadata
5)

Metadata

Metadata

Assignees

No one assigned

    Labels

    No labels
    No labels

    Type

    No type

    Projects

    No projects

    Milestone

    No milestone

    Relationships

    None yet

    Development

    No branches or pull requests

    Issue actions