Problems with the "run scaffold analysis" step

[AbbyKimball]

Hello!

Everything has gone well with my first stab at scaffold so far, but I've run into the same issue three times now. All the data has been processed under the "run clustering" tab, but then I move to the "run scaffold analysis" step and select the first fcs file and the page goes dark and says it's processing, but it just stays like this for over an hour... anyone else experience this issue? Does this step usually take a long time? Thanks!

[AbbyKimball]

Do I need the reference dataset used in Spitzer et al 2015?

[pfgherardini]

Apologies for the late reply. Did you manage to solve the problem? If not I can help.

[AbbyKimball]

Nope! I was never able to resolve the issue. Thanks :)

[pfgherardini]

If you are still interested and give me more information about what you are trying to do I can help you out