[WIP] Analyze endometrial NK cell subtypes using single-cell dataset

[Copilot]

Thanks for asking me to work on this. I will get started on it and keep this PR's description up to date as I form a plan and make progress.

我有一篇文章准备投稿，主要利用子宫内膜单细胞数据集，分析了子宫内膜NK细胞亚群，这是摘要：Abstract Aims Natural killer (NK) cells constitute the dominant immune cell population in the human endometrium, essential for tissue homeostasis and cyclical remodeling. However, the functional heterogeneity and temporal dynamics among uterine (u)NK subtypes across the menstrual cycle remain insufficiently understood. This study aimed to characterize the abundance dynamics and functional specialization of recently designated subtypes (uNK1-3) and to investigate their roles in endometrial disease pathophysiology, particularly endometriosis. Methods We conducted a combined bioinformatic analysis to investigate the transcriptomic and immune landscape of the endometrium across menstrual cycle phases. Transcriptomic shifts were evaluated using four independent bulk RNA-sequencing (RNA-seq) datasets containing 76 pre-receptive and receptive endometrial samples. Abundance variations and functional differences of uNK subtypes were assessed using a single-cell RNA-sequencing (scRNA-seq) dataset comprising endometrial biopsies from 63 donors. Subtype-specific transcriptomic differences in the eutopic endometrium of patients with endometriosis and healthy controls were analyzed using the same scRNA-seq dataset. Key findings Significant shifts in the endometrial immune landscape were observed across menstrual cycle phases. uNK1 cells showed increased abundance during the mid-secretory phase, while uNK2 and uNK3 remained stable. Functional analyses revealed distinct subtype-specific roles, with uNK1 cells displaying cytotoxic potential and uNK3 cells exhibiting enriched cytokine-cytokine receptor interactions. Moreover, dysregulated uNK1 function, characterized by enhanced cytotoxic activity during the proliferative phase, may contribute to endometriosis pathophysiology. Significance This study delineates the dynamic landscape and functional heterogeneity of uNK cell subtypes during endometrial cycling and offers new insights into immune dysregulation underlying endometriosis. Key words: endometrium; immune; natural killer cells; endometriosis; transcriptomics 我现在想要投稿药学相关的期刊，想做一些药学、药物靶标相关的分析，这个分析打算针对我的NK细胞亚群展开。请帮我为进一步分析和生成新的图片做规划： 1. 模拟以上代码的分析方法； 2. 针对NK细胞的亚群做相关药物靶标分析； 3. 写出具体的分析目的、分析流程； 4. 需要一张figure的工作量

---

🔒 GitHub Advanced Security automatically protects Copilot coding agent pull requests. You can protect all pull requests by enabling Advanced Security for your repositories. Learn more about Advanced Security.