• DNA-Seq
  • Introduction
  • Some important links
  • Statistical methods used for analysis

DNA-Seq

Contribution by: @jahnavibhask, @senait2018 and @dktanwar

Introduction

1. DNA sequencing is a technology that attempts to find the order of nucleotides that make up the DNA strand based on the complimentarity between the nucleotides - adenine and thymine or cytosine and guanine of complimentary strands form hydrogen bonds between each other.

2. Fluorescent label tagged nucleotides or sequence-specific restriction enzymes can be used to find the sequence of the DNA strand.

3. Applications - can check for mutations (changes in the base sequence) in the genome, identify and categorise organisms based on their genome sequence, for validation of cloning experiments. It does not give expression status but more about the presence or absence of the DNA sequence element.

4. Technologies - Illumina, oxford nanopore, etc

Some important links

• Wikipedia
• Nice blog by @crazyhottommy
• DNA sequencing at 40
• DNA-Sequencing methods by Illumina

Statistical methods used for analysis

• edgeR
• limma